BioRuby 1.4.3 RELEASE NOTES¶ ↑

A lot of changes have been made to the BioRuby 1.4.3 after the version 1.4.2 is released. This document describes important and/or incompatible changes since the BioRuby 1.4.2 release.

For known problems, see KNOWN_ISSUES.rdoc.

New features¶ ↑

Bio::KEGG::KGML¶ ↑

• New class Bio::KEGG::KGML::Graphics for storing a graphics element. In the instance of the class, “coords” attribute is now available.

• New class Bio::KEGG::KGML::Substrate for storing a substrate element.

• New class Bio::KEGG::KGML::Product for storing a product element.

• New method Bio::KEGG::KGML::Reaction#id.

• Improve RDoc documentation.

• Unit tests are added.

• There are incompatible changes. See Incompatible changes below.

Improvements¶ ↑

Portability running on JRuby and Rubinius¶ ↑

Many failures and errors running on JRuby and Rubinius are resolved. Some of them are due to BioRuby bugs, and some of them are due to JRuby or Rubinius bugs. Artem Tarasov reported bugs in BioRuby and submitted bug reports to Rubinius. Clayton Wheeler and Naohisa Goto fixed bugs in BioRuby and submitted bug reports to JRuby.

Testing on Travis CI¶ ↑

BioRuby is now using Travis CI (travis-ci.org/), a hosted continuous integration service for the open source community.

Bug fixes¶ ↑

Strange behavior related with “circular require” is fixed¶ ↑

Fixed: In previous versions, some bioruby files may be required more than two times, and this sometimes causes strange behavior, depending on the order of files in the disk. In particular, unit tests running on JRuby sometimes crashes with strange errors. In BioRuby 1.4.3, almost all require and autoload lines are revised and are changed to avoid circular require. This also fixes crash on JRuby due to JRuby’s autoload bug.

Other bug fixes¶ ↑

• Fixed: Genomenet remote BLAST does not work.

• Fixed: Bio::KEGG::KGML ignores “coords” field.

• Fixed: Bio::NucleicAcid.to_re(“s”) typo

• To suppress rare failure of chi-square equiprobability tests for Bio::Sequence::Common#randomize, test code changed to retry up to 10 times if the chi-square test fails. The assertion fails if the chi-square test fails 10 consecutive times, and this strongly suggests bugs in codes or in the random number generator.

• Fixed: Bio::EMBL#os raises RuntimeError. The fix includes incompatible change. See below “Incompatible changes”.

• Fixed: bin/bioruby: Failed to save object with error message “can’t convert Symbol into String” on Ruby 1.9.

Incompatible changes and removed features¶ ↑

Bio::FlatFile use binmode (binary mode) when opening a file¶ ↑

In Bio::FlatFile.open and Bio::FlatFile.auto, binmode (binary mode) is used by default when opening a file, unless text mode is explicitly specified with open mode string or with options. Due to the change, files using CR+LF line separator might not be read correctly.

Broader FASTQ file recognition¶ ↑

Because PacBio RS sequencer may produce kilobases long reads and read buffer size (default 31 lines) for file format detection may not be sufficient to find the second id line starting with “+”, the regular expression for FASTQ is truncated only to check the first id line starting with “@”.

Bio::KEGG::KGML¶ ↑

• Bio::KEGG::KGML::Reaction#substrates and Bio::KEGG::KGML::Reaction#products are changed to return an array containing Bio::KEGG::KGML::Substrate and Bio::KEGG::KGML::Product objects, respectively. The changes enables us to get ID of substrates and products that were thrown away in the previous versions.

• Most attribute methods that were different from the KGML attribute names are renamed to the names compatible with the KGML attribute names. Old method names are changed to aliases of them and marked as deprecated. The old names will be removed in the future.

  • Bio::KEGG::KGML::Entry#id (old name: entry_id)

  • Bio::KEGG::KGML::Entry#type (old name: category)

  • Bio::KEGG::KGML::Entry#entry1 (old name: node1)

  • Bio::KEGG::KGML::Entry#entry2 (old name: node2)

  • Bio::KEGG::KGML::Entry#type (old name: rel)

  • Bio::KEGG::KGML::Reaction#name (old name: entry_id)

  • Bio::KEGG::KGML::Reaction#type (old name: direction)

• Following attribute methods are deprecated because two or more graphics elements may exist in an entry element. They will be removed in the future. Instead, please use instance methods of Bio::KEGG::KGML::Graphics, which can be obtained from Bio::KEGG::KGML::Entry#graphics attribute.

  • Bio::KEGG::KGML::Entry#label

  • Bio::KEGG::KGML::Entry#shape

  • Bio::KEGG::KGML::Entry#x

  • Bio::KEGG::KGML::Entry#y

  • Bio::KEGG::KGML::Entry#width

  • Bio::KEGG::KGML::Entry#height

  • Bio::KEGG::KGML::Entry#fgcolor

  • Bio::KEGG::KGML::Entry#bgcolor

Bio::EMBL#os¶ ↑

Bio::EMBL#os, returns parser result of the EMBL OS line, no longer splits the content with comma, and it no longer raises error even if the OS line is not in the “Genus species (name)” format. The changes may affect the parsing of old EMBL files which contain two or more species names in an OS line.

Note that Bio::EMBL#os returns an Array containing several Hash objects, and the argument is always ignored. The return value type and the meaning of the argument might be changed in the future.

Tests¶ ↑

• Tests using network connections are moved under test/network/. To invoke these tests, run “rake test-network”.

• BIORUBY_TEST_LIB environment variable

  • The directory name specified with BIORUBY_TEST_LIB is always added on the top of $LOAD_PATH even if it is already included in the middle of $LOAD_PATH.

  • When BIORUBY_TEST_LIB is empty, it no longer add an empty string to $LOAD_PATH.

  • BIORUBY_TEST_LIB is ignored when BIORUBY_TEST_GEM is set.

• BIORUBY_TEST_GEM environment variable

  • New environment variable BIORUBY_TEST_GEM for testing installed bio-X.X.X gem. Version number can be specified. See the following examples with/without the version number:

    • % env BIORUBY_TEST_GEM=1.4.2.5000 ruby test/runner.rb

    • % env BIORUBY_TEST_GEM=“” ruby test/runner.rb

Other removed features¶ ↑

• rdoc.zsh is removed because it have not been used for a long time.

Known issues¶ ↑

The following issues are added or updated. See KNOWN_ISSUES.rdoc for other already known issues.

JRuby¶ ↑

On JRuby, errors may be raised due to the following unfixed bugs in JRuby.

• JRUBY-6195 Process.spawn (and related methods) ignore option hash

• JRUBY-6818 Kernel.exec, Process.spawn (and IO.popen etc.) raise error when program is an array containing two strings

With older version of JRuby, you may be bothered by the following bugs that have already been fixed in the head of JRuby.

• JRUBY-6658 Problem when setting up an autoload entry, defining a class via require, then redefining the autoload entry

• JRUBY-6666 Open3.popen3 failing due to missing handling for [path, argv] array

• JRUBY-6819 java.lang.ArrayIndexOutOfBoundsException in String#each_line

Due to JRUBY-5678 (resolved issue) and the difference of behavior between CRuby and JRuby written in the comments of the issue tracking page, when running BioRuby on JRuby with sudo or root rights, TMPDIR environment variable should be set to a directory that is not world-writable. Currently, the workaround is needed for running BioRuby tests with JRuby on Travis-CI.

• JRUBY-5678 tmpdir cannot be delete when jruby has sudo/root rights

Rubinius¶ ↑

According to Travis-CI, unit tests have failed on 1.9 mode of Rubinius.

With older version of Rubinius, you may be bothered by the following bugs that have already been fixed in the head of Rubinius.

• Rubinius Issue #1693 String#split gives incorrect output when splitting by /^/

• Rubinius Issue #1724 Creating Struct class with length attribute

DDBJ Web API related classes (Bio::DDBJ::*, Bio::BLAST::Remote::DDBJ)¶ ↑

DDBJ Web API is stopping after their system replacement in March 2012. (See the announcement though it is written only in Japanese: www.ddbj.nig.ac.jp/replace/rp120601-j.html) Due to the stop of the DDBJ Web API, Bio::DDBJ::* and Bio::BLAST::Remote::DDBJ which are using the web API can not be used.

SOAP4R with Ruby 1.9¶ ↑

soap4r-ruby1.9 may raise “ununitialized constant XML::SaxParser” error with some combinations of XML parser libraries. It seems this is a bug of soap4r-ruby1.9.